rabbit polyclonal anti h3  (Cell Signaling Technology Inc)

Journal: Nucleic Acids Research

Article Title: Phosphorylation of Barrier-to-Autointegration Factor (BAF) regulates anchoring of centromeric heterochromatin to the nuclear envelope (NE)

doi: 10.1093/nar/gkag021

Figure Lengend Snippet: Non-phosphorylatable BAF forms delay mitosis. ( A ) The proportion of G2/M cells determined by FACS is shown for S2 cells transiently expressing N-BAF, C-BAF, N-BAF T4A/S5A , or C-BAF T4E/S5E , and for control mock transfected S2 cells. Results are from a single experiment ( N = 3818 for N-BAF, 6565 for C-BAF, 6764 for N-BAF T4A/S5A , 818 for C-BAF T4E/S5E , and 59 729 for control; P -values: ns > .05; * < .05; **** < .0001; Chi-square test). ( B ) Immunostaining with αH3PS10 (in red) of cells transiently expressing N-BAF, C-BAF, or N-BAF T4A/S5A . N-BAF, C-BAF, and N-BAF T4A/S5A signals (in green) are direct fluorescence. DNA was stained with DAPI (in gray). Scale bars are 10 μm. ( C ) Quantification of the results shown in panel (B). The proportion of αH3PS10 positive cells is shown for S2 cells transiently expressing N-BAF, C-BAF, or N-BAF T4A/S5A , and for control mock transfected S2 cells. ( N = 389 for N-BAF, 275 for C-BAF, 235 for N-BAF T4A/S5A , and 748 for control; P -values with respect to control: **** < .0001; Fisher’s exact test).

Article Snippet: The rest of antibodies used are commercially available: mouse monoclonal αHP1a (DSHB C1A9), mouse monoclonal αLaminDm0 (DSHB ADL67.10), rabbit polyclonal αGFP (Invitrogen A11122), mouse polyclonal αGFP (Roche 118144600), rabbit polyclonal αFlag (Merck Life Science F7425), rabbit polyclonal αH3 (Cell Signaling 9715), rabbit polyclonal αH3K9me3 (Millipore 67–442), rabbit polyclonal αH3PS10 (Cell Signaling 9701), and mouse monoclonal αTubulin (MAB3408 Millipore).

Techniques: Expressing, Control, Transfection, Immunostaining, Fluorescence, Staining